Preservation of Coccidial Sporozoites by Freezing

Preservation of Corneal Grafts by Freezing.

Inner ear tissue preservation by rapid freezing: Improving fixation by high-pressure freezing and hybrid methods

In the preservation of tissues in as 'close to life' state as possible, rapid freeze fixation has many benefits over conventional chemical fixation. One technique by which rapid freeze-fixation can be achieved, high pressure freezing (HPF), has been shown to enable ice crystal artefact-free freezing and tissue preservation to greater depths (more than 40 μm) than other quick-freezing methods. D...

Preservation of microbiological assay organisms by direct freezing.

'rhe effects of freezinig on bacterial cultures have been investigated by numerous workers. The most commonly used method of preserving microorganisms is lvophilization (drying from the frozen state), but this is a drastic procedure for in most instances comparatively few organisms (2 to 3 per cent) survive the freeze drying process. Cultures preserved in this way have remained viable for as lo...

Platelet preservation. II. Preservation of canine platelet concentrates by freezing in solutions of glycerol plasma.

Preservation of Mycoplasma strains by freezing in liquid nitrogen and by lyophilization with sucrose.

Procedures for maintaining Mycoplasma strains are described. Liquid nitrogen storage provided an adequate means for keeping stock cultures stable. Over 74 strains of approximately 26 species have been preserved in this way, some for as long as 9 years. Mycoplasma strains can also be recovered satisfactorily from the freeze-dried state when the procedure described includes the use of 12% (v/v) s...